Analysis Note

ControlHuman IL-21 recombinant protein

Application

This Anti-Interleukin 21 (IL-21) Antibody, clone 1G8 is validated for use in WB, ELISA for the detection of Interleukin 21 (IL-21).

ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX)

General description

Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkin′s lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkin′s lymphoma including clusters of other immune cells gathered around HL cells in cultures.

Immunogen

Full length recombinant protein corresponding to human IL-21.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot from human IL-21 recombinant protein.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-21 on 10 µg of human IL-21 recombinant protein.

Target description

Two isoforms are observed at ~16 and ~18 kDa